Cat.No. : CSC-RO0498 Host Cell: CHO-K1
| Cat. No. | CSC-RO0498 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Cyno CD40. |
| Gene | CD40 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: By using the Monkey CD40 Stable Cell Line - CHO-K1, various biochemical and molecular biology experiments can be conducted to study the regulatory mechanisms of the CD40 signaling pathway. For example, immunoprecipitation and mass spectrometry can be used to identify proteins interacting with CD40, and reporter gene analysis can monitor the activity of the CD40 signaling pathway.
A: When conducting gene editing experiments with the Monkey CD40 Stable Cell Line - CHO-K1, it is important to maintain the expression and function of the CD40 protein. The choice and design of gene editing tools, such as CRISPR/Cas9, should ensure specificity to the target gene and accurate validation of the editing effects.
A: The Monkey CD40 Stable Cell Line - CHO-K1 has promising prospects in tumor immunotherapy research. As a co-stimulatory molecule, CD40 plays an important role in regulating anti-tumor immune responses. This cell line can be used to study the role of CD40 in the tumor microenvironment, providing an important experimental platform for developing new immunotherapeutic strategies.
A: The Monkey CD40 Stable Cell Line - CHO-K1 has potential applications in studying B cell-related diseases. As CD40 plays a key role in the activation and proliferation of B cells, this cell line can be used to study the regulatory mechanisms of CD40 in B cell-related diseases, such as autoimmune diseases and certain types of cancers.
A: For antibody screening, experiments should be designed to evaluate the binding capacity and functional impact of different antibodies on the CD40 protein in the Monkey CD40 Stable Cell Line - CHO-K1. ELISA, flow cytometry, or functional cell assays can be used to assess the efficiency of antibody binding and the modulation of the CD40 signaling pathway.
A: The Monkey CD40 Stable Cell Line - CHO-K1, with stable expression of the monkey CD40 receptor, has significant potential in immune regulation research. CD40 is a crucial immunoregulatory molecule that promotes the activation and proliferation of B cells. This cell line can be used to study the role of CD40 in immune responses and develop immunotherapeutic strategies targeting CD40.
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