MECP2-SaCas9HF AAV (Serotype 9)

Name: MECP2-SaCas9HF AAV (Serotype 9)
SKU: AAV00367Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00367Z

Serotype : AAV Serotype 9 Storage : -80 ℃

Titer: Size:

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Virus Particles Information

Quality Control

Cat. No.	AAV00367Z
Description	Premade AAV particles in serotype 9 express high-fidelity Staphylococcus aureus Cas9 (SaCas9) from the MECP2 promoter.
Gene	SaCas9HF
Serotype	AAV Serotype 9
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Background

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Customer Reviews

Adeno-associated virus (AAV) vectors, especially AAV type 9, have become key vectors for gene therapy due to their ability to efficiently deliver genetic material to a variety of tissues. MECP2-SaCas9HF AAV (type 9) is specifically designed to express high-fidelity Staphylococcus aureus Cas9 (SaCas9). The system utilizes the MECP2 promoter, which is the methyl CpG binding protein 2 (Mecp2) promoter, a short, neuron-specific promoter.

The SaCas9 protein derived from Staphylococcus aureus is a smaller but highly efficient alternative to the traditional Streptococcus pyogenes Cas9. Its smaller size makes it ideal for delivery via AAV vectors, which have limited packaging capacity. The high-fidelity (HF) variant further enhances its specificity and reduces off-target effects. This precision is critical in the therapeutic field, especially when editing genes in the human genome to avoid unintended mutations that could cause harmful effects.

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Customer Reviews

Reproducibility

I was impressed with how reproducible the results were across different trials using the EF1a-eNpHR 3.0-EYFP AAV (Serotype 9). This product is a game changer for our research.

Germany

2021-07-19

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MECP2-SaCas9HF AAV (Serotype 9)

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Reproducibility

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