EFS-SaCas9HF AAV (Serotype 9)

Name: EFS-SaCas9HF AAV (Serotype 9)
SKU: AAV00349Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00349Z

Serotype : AAV Serotype 9 Storage : -80 ℃

Titer: Size:

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Virus Particles Information

Quality Control

Cat. No.	AAV00349Z
Description	Premade AAV particles in serotype 9 express high-fidelity Staphylococcus aureus Cas9 (SaCas9) from the EFS promoter.
Gene	SaCas9HF
Serotype	AAV Serotype 9
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Customer Reviews

EFS-SaCas9HF AAV (serotype 9) represents a significant advancement in gene editing technology, providing researchers with a precise and targeted gene modification tool. This prepackaged adeno-associated virus (AAV) is genetically modified with serotype 9 and is specifically designed to express high-fidelity Staphylococcus aureus Cas9 (SaCas9) under the control of the elongation factor 1 short promoter (EFS). The choice of SaCas9 over the traditionally used Streptococcus pyogenes Cas9 (SpCas9) is strategic because SaCas9 is significantly smaller in size, facilitating packaging into AAV vectors with limited genetic material capacity.

AAV serotype 9 is able to efficiently transduce a variety of tissues, including muscle, liver, and central nervous system tissues, making it highly versatile for both in vivo and in vitro applications. The high-fidelity (HF) SaCas9 variant incorporated into these particles ensures greater gene editing precision, minimizing potential off-target effects during CRISPR-Cas9-mediated genome modification. This precision is crucial for applications such as therapeutic gene editing, where unintended genetic changes can have serious consequences.

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Customer Reviews

Exceeded my expectations

An exceptional product for gene editing. The high fidelity and specificity of the SaCas9HF plasmids ensured minimal off-target effects.

Germany

2023-03-30

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EFS-SaCas9HF AAV (Serotype 9)

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Exceeded my expectations

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