Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : HCPAK-02
| Cat. No. | HCPAK-02 |
| Description | This kit is intended for use in determining the presence of host cell protein contamination in products manufactured by recombinant expression in Pichia pastoris. This kit has a very broad reactivity to peptide epitopes and in addition, it has an additional affinity adsorption step to remove antibodies to glycosylation epitopes and is much more specific. |
| Sample Type | Protein |
| Features |
· High sensitivity · Robust, simple assay protocol · Specificity · Easy to use |
| Applications | Determining the presence of Pichia pastoris host cell protein contamination in biological products |
| Method | ELISA |
| Storage | All reagents should be stored at 2℃ to 8℃ for stability until the expiration date printed on the kit. |
A: Host cell proteins mainly include proteins secreted by host cells and some structural proteins produced by cell apoptosis and metabolism.
A: Pichia pastoris expression system has been widely used in the expression of foreign genes, and has achieved great success. Compared with other expression systems, Pichia pastoris expression system has low requirements for nutrients, simple genome structure and can be used for high-density fermentation. These advantages greatly promote the expression of foreign genes.
A: Yes. Pichia pastoris expression system has a strong promoter of AOX, and the expression of foreign gene products is high, which can reach the level of several grams of expression products per liter.
A: Yeast culture, transformation, high-density fermentation and other operations are close to prokaryotic organisms, far simpler than eukaryotic systems, and very suitable for large-scale industrial production.
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Pichia pastoris HCP Assay Kit provides a cost-effective solution to reduce the cost of each test.
The Pichia pastoris HCP Assay Kit is easy to operate, and the test results of the same batch have little difference. Good product.
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