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Pichia pastoris HCP Assay Kit

For research use only. Not intended for any clinical use.
Cat.No.
HCPAK-02
Description
This kit is intended for use in determining the presence of host cell protein contamination in products manufactured by recombinant expression in Pichia pastoris. This kit has a very broad reactivity to peptide epitopes and in addition, it has an additional affinity adsorption step to remove antibodies to glycosylation epitopes and is much more specific.
Applications
Determining the presence of Pichia pastoris host cell protein contamination in biological products
Features
High sensitivity
Robust, simple assay protocol
Specificity
Easy to use
Method
ELISA
Sample Type
Protein
Storage
All reagents should be stored at 2℃ to 8℃ for stability until the expiration date printed on the kit.

Publications

Q & A

Customer Reviews

Customer Q&As
What is the main source of host cell protein?

A: Host cell proteins mainly include proteins secreted by host cells and some structural proteins produced by cell apoptosis and metabolism.

What is Pichia pastoris expression system?

A: Pichia pastoris expression system has been widely used in the expression of foreign genes, and has achieved great success. Compared with other expression systems, Pichia pastoris expression system has low requirements for nutrients, simple genome structure and can be used for high-density fermentation. These advantages greatly promote the expression of foreign genes.

Is the protein yield of the Pichia pastoris expression system high?

A: Yes. Pichia pastoris expression system has a strong promoter of AOX, and the expression of foreign gene products is high, which can reach the level of several grams of expression products per liter.

Is the Pichia pastoris expression system suitable for large-scale industrial production of protein?

A: Yeast culture, transformation, high-density fermentation and other operations are close to prokaryotic organisms, far simpler than eukaryotic systems, and very suitable for large-scale industrial production.

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Customer Reviews
Cost-effective

Pichia pastoris HCP Assay Kit provides a cost-effective solution to reduce the cost of each test.

United States

09/10/2022

Easy to operate

The Pichia pastoris HCP Assay Kit is easy to operate, and the test results of the same batch have little difference. Good product.

United States

01/18/2023

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