Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : HCPAK-04
| Cat. No. | HCPAK-04 |
| Description | The kit is a complete set of optimized reagents that is developed use broadly reactive antibody to HEK293 HCPs and is intended for using in determining the presence of host cell protein impurities in products manufactured by expression in HEK293 host cells. |
| Sample Type | Protein |
| Features |
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| Applications | Determining the presence of HEK293 host cell protein contamination in biological products |
| Method | ELISA |
| Storage | If the kit will not be utilized within 1 week after receipt it is recommended to store the standards at -10°C to -30°C. All other reagents should be stored at 2°C to 8°C for stability until the expiration date printed on the kit. |
A: HEK293 cell line was established by transforming human embryonic kidney cells with cleaved adenovirus type 5 DNA. Since then, many subtypes and derivatives have been established, of which HEK293, HEK293-T and HEK293-F are often used in the production of biopharmaceuticals.
A: The use of HEK293 host cells eliminates the potential immunogenicity problems due to non-human premenstrual metastasis. Its easy transfection and relatively high protein productivity make it a popular choice for small-scale production of recombinant protein scientific research and its ability to adapt to suspension free culture to promote its use in large-scale biotherapy production.
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This kit helps me accurately monitor the change of product concentration and the removal of host cell protein after purification. I recommend it.
I tested up to 37 samples, but it took less than 3 hours. I like the kit.
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