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NS0 HCP Assay Kit

For research use only. Not intended for any clinical use.
Cat.No.
HCPAK-07
Description
The kit is a complete set of optimized reagents that is developed using broadly reactive antibodies to NS0 HCPs and is intended for use in determining the presence of host cell protein contamination in products manufactured by expression in NS0 host cells.
Applications
Determining the presence of NS0 host cell protein contamination in biological products
Features
High sensitivity
Robust, simple assay protocol
Specificity
Easy to use
Method
ELISA
Sample Type
Protein
Storage
Store standards at ≤ -20℃. All other reagents should be stored at 2℃ to 8℃ for stability until the expiration date printed on the kit.

Publications

Q & A

Customer Reviews

Customer Q&As
What is the host cell protein assay development process?

A: The process of a holistic method for HCP assay development begins with the definition/selection of a suitable antigen that can be characterized and conditioned before it gets injected into goats, rabbits, or chickens. In the process of immunization, the animals are thoroughly checked for titer and the specific immune response. The specific anti-HCP antibodies are purified from the antisera and characterized by 2D gel/Western blot for the linear coverage. Ideally, it is characterized by pull-down affinity experiments and mass spectrometry readout. The characterized antibody pool is used for the setup and optimization of the immunoassay, which can be verified and used in routine lot release testing.

What is NS0 cell?

A: NS0 is a mouse myeloma cell that does not synthesize and secrete immunoglobulin heavy chain or light chain. It can express monoclonal antibodies and recombinant proteins using a wide range of screening systems and promoters.

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Customer Reviews
Useful Tool

The presence of residual host proteins may affect the efficacy of protein drugs. This NS0 HCP Assay Kit can help us avoid such problems.

United States

01/18/2023

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