Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : HCPAK-07
| Cat. No. | HCPAK-07 |
| Description | The kit is a complete set of optimized reagents that is developed using broadly reactive antibodies to NS0 HCPs and is intended for use in determining the presence of host cell protein contamination in products manufactured by expression in NS0 host cells. |
| Sample Type | Protein |
| Features |
· High sensitivity · Robust, simple assay protocol · Specificity · Easy to use |
| Applications | Determining the presence of NS0 host cell protein contamination in biological products |
| Method | ELISA |
| Storage | Store standards at ≤ -20℃. All other reagents should be stored at 2℃ to 8℃ for stability until the expiration date printed on the kit. |
A: The process of a holistic method for HCP assay development begins with the definition/selection of a suitable antigen that can be characterized and conditioned before it gets injected into goats, rabbits, or chickens. In the process of immunization, the animals are thoroughly checked for titer and the specific immune response. The specific anti-HCP antibodies are purified from the antisera and characterized by 2D gel/Western blot for the linear coverage. Ideally, it is characterized by pull-down affinity experiments and mass spectrometry readout. The characterized antibody pool is used for the setup and optimization of the immunoassay, which can be verified and used in routine lot release testing.
A: NS0 is a mouse myeloma cell that does not synthesize and secrete immunoglobulin heavy chain or light chain. It can express monoclonal antibodies and recombinant proteins using a wide range of screening systems and promoters.
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The presence of residual host proteins may affect the efficacy of protein drugs. This NS0 HCP Assay Kit can help us avoid such problems.
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