Cat.No. : CSC-RO0434 Host Cell: CHO-K1
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0434 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Human ROR1. |
| Target Gene | ROR1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is a promising target for cancer immunotherapy, as it is overexpressed in various hematopoietic and solid tumors. In this study, researchers evaluated different ROR1 fusion proteins in preclinical models to assess their potential as cancer vaccines. They developed fusion proteins combining mouse ROR1, tetanus toxin epitopes, and the Fc region of mouse IgG2a to break immune tolerance and stimulate anti-tumor immunity. The results demonstrated that immunization with these fusion proteins significantly enhanced immune responses, including the induction of ROR1-specific antibodies and a strong cytotoxic T lymphocyte (CTL) response. Most importantly, the immunized mice showed complete inhibition of ROR1+ tumor growth.
Figure 1. The researchers assessed the reactivity of rabbit anti-mROR1 antibodies and characterized the fusion proteins using SDS-PAGE under both reducing and non-reducing conditions. (Hassannia H, et al., 2022)
Creative Biogene's Human ROR1 Stable Cell Line - CHO-K1 can serve as an effective tool for similar cancer immunotherapy research. This cell line can be used to produce ROR1 fusion proteins or other recombinant antigens, helping researchers explore novel cancer vaccine strategies.
A: The impact of the CHO-K1 cell line as a vector on the Human ROR1 stable cell line is believed to be minimal. However, we recommend that customers pay attention to the special growth requirements of the CHO-K1 cell line during use to ensure optimal expression and stability of the cells. We provide detailed training guides to help clients overcome potential issues.
A: Our Human ROR1 stable cell line exhibits a high degree of stability in long-term culture. We verified the stability of gene expression through PCR, Western blot and other methods, and conducted repeated tests at different time points and in different batches. The results showed that the cell line expression was stable with minimal fluctuations in gene expression levels.
A: Yes, our CHO-K1 cell line has undergone a rigorous screening and optimization process. We use specific screening criteria, including antibiotic selection and gene expression levels, to ensure that the stability and expression levels of the cell lines meet our quality standards. We also performed long-term culture and expansion of the cell line to verify its stability and reproducibility under different conditions.
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In my experiments, the cells showed a continuous growth trend, which provided a reliable data basis for my research. The cells behaved very consistently both in the early stages of cell culture and during subsequent experiments, which made my experimental results more credible.
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