Human KRAS Stable Cell Line - NIH/3T3

Name: Human KRAS Stable Cell Line - NIH/3T3
SKU: CSC-RO0594
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0594

Host Cell : NIH3T3 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0594
Description	This cell line is derived from NIH_3T3 and is engineered to stably overexpress Human KRAS.
Target Gene	KRAS
Gene Species	Homo sapiens (Human)
Host Cell	NIH3T3
Host Cell Species	Mus musculus (Mouse)
Applications	Drug screening and biological assays
Size	>1x10^6 frozen cells/vial, 1 mL
Stability	Stable in culture over a minimum of 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KRAS v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog [ Homo sapiens ]
Gene Symbol	KRAS
Synonyms	NS; NS3; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Gene Description	v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene ID	3845
Uni Prot ID	P01116
m RNA Refseq	NM_033360.2
Protein Refseq	NP_203524.1
Chromosome Location	12p12.1
Function	GDP binding; GMP binding; GTP binding; GTPase activity; LRR domain binding; protein binding;
Pathway	ARMS-mediated activation, organism-specific biosystem; Activation of RAS in B Cells, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem;
MIM	190070

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