Cat.No. : CSC-RO0567 Host Cell: HEK293T
| Cat. No. | CSC-RO0567 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human ILDR2. |
| Gene | ILDR2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The advantage of the Human ILDR2 Stable Cell Line - HEK293T in drug screening lies in its stable expression of the ILDR2 protein, which is crucial for testing drugs targeting ILDR2. Given the high transfection efficiency and ease of cultivation of HEK293T cells, they can be used efficiently in large-scale drug screening experiments, accelerating drug development.
A: The intracellular pathway of ILDR2 in Human ILDR2 Stable Cell Line - HEK293T can be studied by transfecting specific reporter genes or using proteomic approaches. Observing the effects of ILDR2 activation, inhibition, or mutation on cellular signaling, metabolism, and phenotype can reveal its mechanisms of action within the cell.
A: The rapid proliferation and higher genetic instability of HEK293T cells should be considered in gene editing experiments. Therefore, when designing CRISPR/Cas9 or other gene editing experiments, it is important to select appropriate targets and perform thorough clone selection and validation to ensure precision and effectiveness.
A: This cell line has broad prospects in simulating specific disease environments, such as cancer and autoimmune diseases. Studying the function and regulatory mechanisms of ILDR2 can enhance our understanding of the pathogenesis of these diseases and provide an important cellular model for new drug development.
A: To improve the efficiency of high-throughput screening, optimizing culture conditions, transfection efficiency, and screening methods is essential. Adjusting cell density, using efficient transfection reagents and screening platforms, and employing automated data collection and analysis systems can enhance the speed and accuracy of screening.
A: In immune regulation research, the Human ILDR2 Stable Cell Line - HEK293T can be used to study the role of the ILDR2 protein in immune responses. Especially in studying T-cell activation, inhibition, and immune escape mechanisms, this cell line provides a valuable tool for unraveling the complex regulatory network of the immune system.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
