Human ILDR2 Stable Cell Line - HEK293T

Name: Human ILDR2 Stable Cell Line - HEK293T
SKU: CSC-RO0567
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0567

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0567
Description	This cell line is derived from HEK293T and is engineered to stably overexpress Human ILDR2.
Target Gene	ILDR2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ILDR2 immunoglobulin-like domain containing receptor 2 [ Homo sapiens ]
Gene Symbol	ILDR2
Synonyms	C1orf32; dJ782G3.1; RP4-782G3.2
Gene ID	387597
Uni Prot ID	Q71H61
m RNA Refseq	NM_199351.2
Protein Refseq	NP_955383.1
Chromosome Location	1q24.1

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What are the potential advantages of the Human ILDR2 Stable Cell Line - HEK293T in drug screening?

A: The advantage of the Human ILDR2 Stable Cell Line - HEK293T in drug screening lies in its stable expression of the ILDR2 protein, which is crucial for testing drugs targeting ILDR2. Given the high transfection efficiency and ease of cultivation of HEK293T cells, they can be used efficiently in large-scale drug screening experiments, accelerating drug development.

How can the intracellular pathway of ILDR2 protein be studied using Human ILDR2 Stable Cell Line - HEK293T?

A: The intracellular pathway of ILDR2 in Human ILDR2 Stable Cell Line - HEK293T can be studied by transfecting specific reporter genes or using proteomic approaches. Observing the effects of ILDR2 activation, inhibition, or mutation on cellular signaling, metabolism, and phenotype can reveal its mechanisms of action within the cell.

What special considerations should be taken into account when using Human ILDR2 Stable Cell Line - HEK293T for gene editing experiments?

A: The rapid proliferation and higher genetic instability of HEK293T cells should be considered in gene editing experiments. Therefore, when designing CRISPR/Cas9 or other gene editing experiments, it is important to select appropriate targets and perform thorough clone selection and validation to ensure precision and effectiveness.

What are the prospects of using Human ILDR2 Stable Cell Line - HEK293T in simulating disease environments?

A: This cell line has broad prospects in simulating specific disease environments, such as cancer and autoimmune diseases. Studying the function and regulatory mechanisms of ILDR2 can enhance our understanding of the pathogenesis of these diseases and provide an important cellular model for new drug development.

How can experimental conditions be optimized to improve screening efficiency when using Human ILDR2 Stable Cell Line - HEK293T for high-throughput screening?

A: To improve the efficiency of high-throughput screening, optimizing culture conditions, transfection efficiency, and screening methods is essential. Adjusting cell density, using efficient transfection reagents and screening platforms, and employing automated data collection and analysis systems can enhance the speed and accuracy of screening.

What is the potential role of Human ILDR2 Stable Cell Line - HEK293T in studying immune regulation?

A: In immune regulation research, the Human ILDR2 Stable Cell Line - HEK293T can be used to study the role of the ILDR2 protein in immune responses. Especially in studying T-cell activation, inhibition, and immune escape mechanisms, this cell line provides a valuable tool for unraveling the complex regulatory network of the immune system.

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