Human CACNA1C Knockout Cell Line-HepG2

Name: Human CACNA1C Knockout Cell Line-HepG2
SKU: CSC-RT2627
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2627

Target Gene : CACNA1C Host Cell : HepG2

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2627
Description	This cell is a stable cell line with a homozygous knockout of human CACNA1C using CRISPR/Cas9.
Target Gene	CACNA1C
Host Cell	HepG2
Host Cell Species	Homo sapiens (Human)
Size	1 vial (10^6 cell/vial)
Validation	Sequencing
Storage	Liquid nirtogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CACNA1C calcium channel, voltage-dependent, L type, alpha 1C subunit [ Homo sapiens ]
Gene Symbol	CACNA1C
Synonyms	TS; LQT8; CACH2; CACN2; CaV1.2; CCHL1A1; CACNL1A1
Gene Description	calcium channel, voltage-dependent, L type, alpha 1C subunit
GeneID	775
Uni ProtID	Q13936
mRNA Refseq	NM_001129838.1
Protein Refseq	NP_001123310.1
Chromosome Location	12p13.3
Function	alpha-actinin binding; calmodulin binding; protein binding; protein domain specific binding; protein phosphatase 2A binding; voltage-gated calcium channel activity;
Pathway	Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Alzheimers Disease, organism-specific biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Arrhythmogenic right ventricular cardiomyopathy, organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem;
MIM	114205

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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