human SMAD3 (NM_001145104) lentivirus particles

Name: human SMAD3 (NM_001145104) lentivirus particles
SKU: LV26066L
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV26066L

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	LV26066L
Description	Lentiviral particles of SMAD family member 3 (SMAD3), transcript variant 4.
Product Type	Premade Lentivirus Particles
Gene	SMAD3
Species	Human
Insert Size	693 bp
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	SMAD3 SMAD family member 3 [ Homo sapiens ]
Gene Symbol	SMAD3
Synonyms	LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Gene Description	SMAD family member 3
GeneID	4088
Uni ProtID	P84022
mRNA Refseq	NM_005902.3
Protein Refseq	NP_005893.1
Chromosome Location	15q22.33
Function	R-SMAD binding; RNA polymerase II activating transcription factor binding; beta-catenin binding; chromatin DNA binding; co-SMAD binding; collagen binding; core promoter proximal region sequence-specific DNA binding; double-stranded DNA binding; phosphatase binding; protein binding; contributes_to protein binding transcription factor activity; protein homodimerization activity; protein kinase binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; contributes_to sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription regulatory region DNA binding; transforming growth factor beta receptor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin binding; ubiquitin protein ligase binding; zinc ion binding;
Pathway	Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Adipogenesis, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem;
MIM	603109

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