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human GLI3 (NM_000168) lentivirus particles

For research use only. Not intended for any clinical use.

Cat. No. :   LV13666L

Storage :   -80℃ Shipping :   Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No. LV13666L
Description Lentiviral particles of GLI family zinc finger 3 (GLI3).
Product Type Premade Lentivirus Particles
Gene GLI3
Species Human
Insert Size 4743 bp
Titer Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc.
Size Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Frozen on dry ice
Summary Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.
Gene Name GLI3 GLI family zinc finger 3 [ Homo sapiens ]
Gene Symbol GLI3
Synonyms GLI3; GLI family zinc finger 3; GCPS, GLI Kruppel family member GLI3 , glioma associated oncogene family zinc finger 3 , Greig cephalopolysyndactyly syndrome , PHS; transcriptional activator GLI3; ACLS; DNA binding protein; oncogene GLI3; PAP A; PAPA; PAPA1; PAPB; PPDIV; zinc finger protein GLI3; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3; PHS; GCPS; PAP-A; GLI3FL; GLI3-190;
Gene ID 2737
Uni Prot ID P10071
m RNA Refseq BC113616
Chromosome Location 7p13
Function beta-catenin binding; chromatin binding; histone acetyltransferase binding; histone deacetylase binding; metal ion binding
Pathway Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Endochondral Ossification, organism-specific biosystem; Hedgehog Signaling Pathway, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem; Hedgehog signaling pathway, organism-specific biosystem; Hedgehog signaling pathway, conserved biosystem;
MIM 165240
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