Panoply™ Human GLI3 Over-expressing Stable Cell Line

Name: Panoply™ Human GLI3 Over-expressing Stable Cell Line
SKU: CSC-SC006316
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC006316

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC006316
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	GLI3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GLI3 GLI family zinc finger 3 [ Homo sapiens ]
Gene Symbol	GLI3
Synonyms	GLI3; GLI family zinc finger 3; GCPS, GLI Kruppel family member GLI3 , glioma associated oncogene family zinc finger 3 , Greig cephalopolysyndactyly syndrome , PHS; transcriptional activator GLI3; ACLS; DNA binding protein; oncogene GLI3; PAP A; PAPA; PAPA1; PAPB; PPDIV; zinc finger protein GLI3; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3; PHS; GCPS; PAP-A; GLI3FL; GLI3-190;
Gene ID	2737
Uni Prot ID	P10071
m RNA Refseq	BC113616
Chromosome Location	7p13
Function	beta-catenin binding; chromatin binding; histone acetyltransferase binding; histone deacetylase binding; metal ion binding
Pathway	Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Endochondral Ossification, organism-specific biosystem; Hedgehog Signaling Pathway, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem; Hedgehog signaling pathway, organism-specific biosystem; Hedgehog signaling pathway, conserved biosystem;
MIM	165240

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