human EGLN1 (NM_022051) lentivirus particles

Name: human EGLN1 (NM_022051) lentivirus particles
SKU: LV11510L
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV11510L

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	LV11510L
Description	Lentiviral particles of egl nine homolog 1 (C. elegans) (EGLN1).
Product Type	Premade Lentivirus Particles
Gene	EGLN1
Species	Human
Insert Size	1281 bp
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	EGLN1 egl nine homolog 1 (C. elegans) [ Homo sapiens ]
Gene Symbol	EGLN1
Synonyms	HPH2; PHD2; SM20; ECYT3; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2
Gene Description	egl nine homolog 1 (C. elegans)
Gene ID	54583
Uni Prot ID	Q9GZT9
m RNA Refseq	NM_022051.2
Protein Refseq	NP_071334.1
Chromosome Location	1q42.1
Function	L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; peptidyl-proline 4-dioxygenase activity; peptidyl-proline dioxygenase activity; protein binding; zinc ion binding;
Pathway	Cellular response to hypoxia, organism-specific biosystem; Cellular responses to stress, organism-specific biosystem; HIF-1 signaling pathway, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem; Oxygen-dependent Proline Hydroxylation of Hypoxia-inducible Factor Alpha, organism-specific biosystem; Pathways in cancer, organism-specific biosystem;
MIM	606425

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