Panoply™ Human EGLN1 Knockdown Stable Cell Line

Name: Panoply™ Human EGLN1 Knockdown Stable Cell Line
SKU: CSC-DC004773
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC004773

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-DC004773
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	EGLN1
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	EGLN1 egl nine homolog 1 (C. elegans) [ Homo sapiens ]
Gene Symbol	EGLN1
Synonyms	HPH2; PHD2; SM20; ECYT3; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2
Gene Description	egl nine homolog 1 (C. elegans)
Gene ID	54583
Uni Prot ID	Q9GZT9
m RNA Refseq	NM_022051.2
Protein Refseq	NP_071334.1
Chromosome Location	1q42.1
Function	L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; peptidyl-proline 4-dioxygenase activity; peptidyl-proline dioxygenase activity; protein binding; zinc ion binding;
Pathway	Cellular response to hypoxia, organism-specific biosystem; Cellular responses to stress, organism-specific biosystem; HIF-1 signaling pathway, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem; Oxygen-dependent Proline Hydroxylation of Hypoxia-inducible Factor Alpha, organism-specific biosystem; Pathways in cancer, organism-specific biosystem;
MIM	606425

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