human CDC27 (NM_001114091) lentivirus particles

Name: human CDC27 (NM_001114091) lentivirus particles
SKU: LV08530L
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV08530L

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	LV08530L
Description	Lentiviral particles of cell division cycle 27 homolog (S. cerevisiae) (CDC27), transcript variant 1.
Product Type	Premade Lentivirus Particles
Gene	CDC27
Species	Human
Insert Size	2493 bp
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	CDC27 cell division cycle 27 homolog (S. cerevisiae) [ Homo sapiens ]
Gene Symbol	CDC27
Synonyms	APC3; HNUC; NUC2; ANAPC3; CDC27Hs; D0S1430E; D17S978E
Gene Description	cell division cycle 27 homolog (S. cerevisiae)
GeneID	996
Uni ProtID	P30260
mRNA Refseq	NM_001256.3
Protein Refseq	NP_001247.3
Chromosome Location	17q21.32
Function	protein binding; protein phosphatase binding;
Pathway	APC/C complex, organism-specific biosystem; APC/C complex, conserved biosystem; APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Cyclin B, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem;
MIM	116946

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