Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : PMRNL-0004
| Cat. No. | PMRNL-0004 |
| Description | The Firefly Luciferase (fLuc) mRNA is designed to produce high expression level of FireFly Luciferase which is commonly used in mammalian cell culture to measure both gene expression and cell viability. The incorporation of N1-methylpseudouridine can reduce the immunogenicity of the resulting mRNA. |
| Gene Type | Reporter Gene |
| RNA Type | mRNA |
| Gene Name | FLuc |
| Features |
• mRNA synthesized on error free sequence verified plasmid DNA template • 100% replacement of UTP with modified nucleotides N1-Methylpseudo-UTP • Cap 1 Capping and poly-A tailed incorporated • Degrades the DNA template after RNA synthesis with DNase |
| Sequence | MASKLGSEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVLE |
| Storage | Store at or below -70°C. Avoid repeated freeze/thaw cycles. Aliquot if necessary using RNase-free equipment, reagents, pipet tips, tubes, and containers. |
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