Firefly Luciferase mRNA (ARCA capped, 5-Methyl-CTP & ψUTP modified)

PMRN-0302

The Firefly Luciferase mRNA can express luciferase protein, which has been initially extracted from the Photinus pyralis (Firefly), once it has been transfected into cells. Firefly Luciferase is a commonly used bioluminescent reporter for gene regulation and function study. It is applicable in various assays such as gene expression, cell viability, and in vivo imaging. Anti-Reverse Cap Analog (ARCA) is a cap analog used during in vitro transcription to generate capped transcripts. ARCA obtains the ability only to insert in the proper orientation which ensures ARCA-capped mRNA with high translation efficacy. Meanwhile, the incorporation of 5-Methyl-CTP and ψUTP can reduce the immunogenicity of the resulting mRNA.

• mRNA synthesized on error free sequence verified plasmid DNA template
• ARCA capping and poly-A tail incorporated
• mRNA modified with 5-Methyl-CTP and ψUTP
• Degrades the DNA template after RNA synthesis with DNase

mRNA Length: 1921 base

Store at or below -70°C. Avoid repeated freeze/thaw cycles. Aliquot if necessary using RNase-free equipment, reagents, pipet tips, tubes, and containers.