dCas9-BFP-KRAB Stable Cell Line - HeLa

Name: dCas9-BFP-KRAB Stable Cell Line - HeLa
SKU: CSC-RO0669
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0669 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : T7 Endonuclease I assay

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0669
Description	This cell line is engineered to stably overexpress HA-tagged dCas9 nuclease fused to blue fluorescent protein (BFP) and the transcriptional repression domain Krüppel associated box (KRAB) in HeLa. It is designed for CRISPR interference (CRISPRi) application.
Introduction	Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology that contains two essential components: a guide RNA (gRNA) to match a target gene, and the Cas9 (CRISPR-associated protein 9) endonuclease which causes a double-stranded DNA break, allowing modifications to the genome via nonhomologous end joining (NHEJ) or homology-directed repair (HDR).
Product Type	Cas9 overexpression stable cell line
Target Gene	dCas9-BFP-KRAB
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Applications	Transcriptional inhibition of endogenous genes
Size	One vial of frozen cells, typically >1x10⁶ cells/vial
Validation	T7 Endonuclease I assay
Quality Control	1) Gene inhibition assay 2) Mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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