CMV-FLPo-Puro Lentivirus

Name: CMV-FLPo-Puro Lentivirus
SKU: LV00983Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV00983Z

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Cat. No.	LV00983Z
Description	This lentivirus contains (mouse codon optimized FLPe) FLPo-IRES-Puromycin under the control of CMV promoter.
Gene	FLPo
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

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Lentivirus is a type of retrovirus that requires a relatively long incubation time, so it is called "Lenti" virus. It includes human immunodeficiency virus (HIV), feline immunodeficiency virus (FIV), simian immunodeficiency virus (SIV), bovine immunodeficiency virus, etc. The most studied of these is HIV-1 lentivirus.

Lentivirus vector is based on the lentivirus genome, and is constructed by replacing some genes with the required target gene. Currently used lentivirus vectors are mostly modified from the HIV-1 genome. Compared with general retrovirus vectors, lentivirus vectors have the ability to infect both dividing cells and non-dividing cells and have a wider host range. Lentivirus vectors can also effectively integrate exogenous genes into host chromosomes, thereby achieving persistent expression. In terms of infectivity, it can effectively infect various types of cells such as neurons, hepatocytes, cardiomyocytes, tumor cells, endothelial cells, stem cells, etc., and rarely triggers the body's immune response, which can achieve good gene therapy effects and has broad application prospects.

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Customer Reviews

Consistent Performance in Experiments

The lentivirus provided reproducible results across different batches, which was key to my long-term research projects. Its high consistency helped in achieving smooth progress in my cell transfection trials.

Canada

2022-09-11

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CMV-FLPo-Puro Lentivirus

Virus Particles Information

Quality Control

Background

Q & A

Customer Reviews

Consistent Performance in Experiments

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