Panoply™ Human TSLP Over-expressing Stable Cell Line

Name: Panoply™ Human TSLP Over-expressing Stable Cell Line
SKU: CSC-SC016721
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC016721

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC016721
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	TSLP
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TSLP thymic stromal lymphopoietin [ Homo sapiens ]
Gene Symbol	TSLP
Synonyms	TSLP; thymic stromal lymphopoietin;
GeneID	85480
Uni ProtID	Q969D9
mRNA Refseq	BC016720
Chromosome Location	5q22.1
Function	cytokine activity;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
MIM	607003

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Thymic stromal lymphopoietin (TSLP) plays a significant role in the initiation and progression of various neoplastic diseases. Here, researchers demonstrate that TSLP is aberrantly downregulated in multiple types of cancer compared to normal tissues and correlates with distinct molecular and immune subtypes. ROC curve and survival analyses indicate that TSLP expression is closely associated with diagnosis, prognosis, clinical characteristics, and immune cell infiltration in patients with lung adenocarcinoma (LUAD). In vitro experiments confirm that TSLP overexpression significantly reduces LUAD cell viability, promotes apoptosis, induces G2/M cell cycle arrest, and inhibits cell migration and invasion. Furthermore, TSLP suppresses LUAD progression via the JAK1/STAT3 signaling pathway. Consequently, targeting TSLP represents a potential therapeutic strategy for pan-cancer-particularly LUAD-and serves as a prognostic biomarker for this malignancy.

Here, the researchers investigated the effect of TSLP on tumor metastasis. They performed wound-healing assays using control cells and TSLP-overexpressing cells, assessing cell migration capacity by measuring the extent of wound closure at 24, 48, and 72 hours. The results showed that the wound area was larger in the TSLP-overexpressing group compared to the control group (Figure 1H, I), indicating that TSLP overexpression significantly reduced the migration capacity of A549 cells. Furthermore, Transwell assays demonstrated that TSLP-overexpressing cells exhibited a reduced ability to penetrate Matrigel-coated membranes. The data revealed a significant decrease in the number of cells penetrating the Matrigel matrix in the TSLP-overexpression group compared to the control group, confirming that TSLP overexpression inhibits the invasive capacity of A549 (Figure 1J, K) and NCI-H1299 (Figure 1L, M) cells. Therefore, it can be concluded that TSLP exerts an inhibitory effect on the migration and invasion of lung adenocarcinoma (LUAD) cells.

Figure 1. Overexpression of TSLP inhibited cell proliferation, migration, and invasion. (Qu H, et al., 2023)

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