Panoply™ Human RASGRF1 Over-expressing Stable Cell Line

Name: Panoply™ Human RASGRF1 Over-expressing Stable Cell Line
SKU: CSC-SC012997
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC012997

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC012997
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	RASGRF1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	RASGRF1 Ras protein-specific guanine nucleotide-releasing factor 1 [ Homo sapiens ]
Gene Symbol	RASGRF1
Synonyms	RASGRF1; Ras protein-specific guanine nucleotide-releasing factor 1; GRF1; ras-specific guanine nucleotide-releasing factor 1; CDC25; CDC25L; GNRP; GRF55; H GRF55; PP13187; guanine nucleotide exchange factor; guanine nucleotide-releasing protein; guanine nucleotide-releasing factor 1; guanine nucleotide-releasing factor, 55 kD; Ras-specific nucleotide exchange factor CDC25; Ras-specific guanine nucleotide-releasing factor, CDC25 homolog; H-GRF55; ras-GRF1;
Gene ID	5923
Uni Prot ID	Q13972
m RNA Refseq	BC040275
Chromosome Location	15q24
Pathway	Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; CREB phosphorylation through the activation of Ras, organism-specific biosystem; Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; G alpha (12/13) signalling events, organism-specific biosystem;
MIM	606600

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