Human RASGRF1 adenoviral particles

Name: Human RASGRF1 adenoviral particles
SKU: AD13465Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AD13465Z

Storage : -80℃ Shipping : Frozen on dry ice

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	AD13465Z
Description	Adenovirus with ORF of Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), transcript variant 1.
Gene	RASGRF1
Species	Human
Titer	Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility	Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection	All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays	Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering	All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.

Gene Name	RASGRF1 Ras protein-specific guanine nucleotide-releasing factor 1 [ Homo sapiens ]
Gene Symbol	RASGRF1
Synonyms	RASGRF1; Ras protein-specific guanine nucleotide-releasing factor 1; GRF1; ras-specific guanine nucleotide-releasing factor 1; CDC25; CDC25L; GNRP; GRF55; H GRF55; PP13187; guanine nucleotide exchange factor; guanine nucleotide-releasing protein; guanine nucleotide-releasing factor 1; guanine nucleotide-releasing factor, 55 kD; Ras-specific nucleotide exchange factor CDC25; Ras-specific guanine nucleotide-releasing factor, CDC25 homolog; H-GRF55; ras-GRF1;
Gene ID	5923
Uni Prot ID	Q13972
m RNA Refseq	BC040275
Chromosome Location	15q24
Pathway	Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; CREB phosphorylation through the activation of Ras, organism-specific biosystem; Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; G alpha (12/13) signalling events, organism-specific biosystem;
MIM	606600

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