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Panoply™ Human RASGRF1 Knockdown Stable Cell Line

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-DC012997

Host Cell :   HEK293 (Hela and other cell types are also available) Validation :   Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No. CSC-DC012997
Description Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene RASGRF1
Host Cell HEK293 (Hela and other cell types are also available)
Host Cell Species Homo sapiens (Human)
Applications

(1) Studying gene functions

(2) Studying gene interactions and signaling pathways

(3) Target validation and drug discovery

(4) Designing diseases models

Size >1 × 106 cells / vial
Stability Validated for at least 10 passages
Validation Real-Time RCR
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid Nitrogen
Shipping Dry Ice
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name RASGRF1 Ras protein-specific guanine nucleotide-releasing factor 1 [ Homo sapiens ]
Gene Symbol RASGRF1
Synonyms RASGRF1; Ras protein-specific guanine nucleotide-releasing factor 1; GRF1; ras-specific guanine nucleotide-releasing factor 1; CDC25; CDC25L; GNRP; GRF55; H GRF55; PP13187; guanine nucleotide exchange factor; guanine nucleotide-releasing protein; guanine nucleotide-releasing factor 1; guanine nucleotide-releasing factor, 55 kD; Ras-specific nucleotide exchange factor CDC25; Ras-specific guanine nucleotide-releasing factor, CDC25 homolog; H-GRF55; ras-GRF1;
Gene ID 5923
Uni Prot ID Q13972
m RNA Refseq BC040275
Chromosome Location 15q24
Pathway Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; CREB phosphorylation through the activation of Ras, organism-specific biosystem; Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; G alpha (12/13) signalling events, organism-specific biosystem;
MIM 606600
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