Panoply™ Human MED1 Over-expressing Stable Cell Line

Name: Panoply™ Human MED1 Over-expressing Stable Cell Line
SKU: CSC-SC009352
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC009352

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC009352
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	MED1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MED1 mediator complex subunit 1 [ Homo sapiens ]
Gene Symbol	PPARBP
Synonyms	PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
Gene Description	PPAR binding protein
GeneID	5469
Uni ProtID	Q15648
mRNA Refseq	NM_004774.3
Protein Refseq	NP_004765.2
Chromosome Location	17q12
Function	LBD domain binding; RNA polymerase II transcription cofactor activity; chromatin binding; core promoter binding; estrogen receptor binding; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; ligand-dependent nuclear receptor transcription coactivator activity; mediator complex binding; peroxisome proliferator activated receptor binding; protein binding; receptor activity; retinoic acid receptor binding; sequence-specific DNA binding RNA polymerase II transcription factor activity; thyroid hormone receptor binding; thyroid hormone receptor binding; thyroid hormone receptor coactivator activity; transcription coactivator activity; transcription coactivator activity; transcription cofactor activity; transcription factor binding; vitamin D receptor binding; vitamin D receptor binding; vitamin D receptor binding;
Pathway	Activation of Gene Expression by SREBP (SREBF), organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of Expression by REV-ERBA, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Energy Metabolism, organism-specific biosystem;
MIM	604311

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