Mouse Vsir Stable Cell Line - CHO-K1

Name: Mouse Vsir Stable Cell Line - CHO-K1
SKU: CSC-RO0213
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0213

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0213
Description	This cell line is engineered to stably overexpress the mouse Vsir in CHO-K1 cells.
Introduction	This cell line is constructed by transfection of mouse V-set immunoregulatory receptor (Vsir) into CHO-K1, followed by stable cell selection. The expression of Vsir has been analyzed through flow cytometry.
Target Gene	Vsir
Gene Species	Mus musculus (Mouse)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

What are the key genetic modifications that characterize the Mouse Vsir Stable Cell Line - CHO-K1, and how do these modifications enhance its utility for biotechnology applications?

A: The Mouse Vsir Stable Cell Line - CHO-K1 typically features the introduction of multiple viral integration sites (Viral Integrating Sites or Vsir) that allow for the stable integration of multiple transgenes. These sites are engineered to facilitate the insertion of foreign genes without disrupting the host genome, which is crucial for protein production and gene therapy studies. The genetic modifications also include the use of selectable markers to ensure the cells that have integrated the desired transgenes can be isolated and expanded.

How does the Mouse Vsir Stable Cell Line - CHO-K1 differ from other CHO cell lines in terms of its growth characteristics and protein production efficiency?

A: The Mouse Vsir Stable Cell Line - CHO-K1 is known for its robust growth and high protein production capabilities. Compared to other CHO cell lines, it may exhibit faster growth rates, higher cell density, and potentially better stability of the expressed proteins. These characteristics make it a preferred choice for large-scale biomanufacturing processes.

What are the typical applications of the Mouse Vsir Stable Cell Line - CHO-K1 in the field of biologics production?

A: The Mouse Vsir Stable Cell Line - CHO-K1 is widely used for the production of monoclonal antibodies, recombinant proteins, and other biopharmaceuticals. Its ability to stably integrate and express multiple genes makes it an ideal platform for the development of complex therapeutics, such as bispecific antibodies and multi-subunit proteins.

How does the Mouse Vsir Stable Cell Line - CHO-K1 respond to different culture conditions, and what are the optimal conditions for its growth and protein production?

A: The Mouse Vsir Stable Cell Line - CHO-K1 can adapt to various culture conditions, including serum-free media and different oxygen levels. Optimal growth conditions typically involve a nutrient-rich medium, a controlled temperature of around 37°C, and a pH range of 7.2 to 7.4. For protein production, the cells may require additional supplements, such as growth factors or specific nutrients, depending on the protein being expressed.

How does the Mouse Vsir Stable Cell Line - CHO-K1 contribute to the development of personalized medicine through the production of patient-specific therapeutics?

A: The Mouse Vsir Stable Cell Line - CHO-K1 can be engineered to produce patient-specific antibodies or other therapeutic proteins. This enables the development of personalized treatments, such as customized antibodies for cancer therapy, which can target specific tumor antigens without affecting healthy cells.

How does the Mouse Vsir Stable Cell Line - CHO-K1 support the development of novel vaccine platforms?

A: The Mouse Vsir Stable Cell Line - CHO-K1 can be used to produce antigens or adjuvants for vaccine development. Its high protein production capabilities allow for the efficient production of these components, which can then be formulated into vaccines for various infectious diseases.

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