Mouse Hmgb1 HEK293 Cell Lysate

Name: Mouse Hmgb1 HEK293 Cell Lysate
SKU: CLOE-2406
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-2406 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-2406
Introduction	This cell lysate over-expresses mouse Hmgb1 (P63158) (Met 1-Glu 215) fused with human IgG1-Fc at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	Hmgb1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	Hmgb1 high mobility group box 1 [ Mus musculus ]
Gene Symbol	Hmgb1
Synonyms	DEF; p30; Hmg1; HMG-1; SBP-1; amphoterin
Gene Description	high mobility group box 1
Gene ID	15289
Uni Prot ID	P63158
m RNA Refseq	NM_010439.3
Protein Refseq	NP_034569.1
Chromosome Location	5 G3; 5 83.0 cM
Function	5S rRNA binding; DNA binding; DNA binding, bending; RAGE receptor binding; bent DNA binding; calcium-dependent protein kinase regulator activity; crossed form four-way junction DNA binding; cytokine activity; cytokine activity; double-stranded DNA binding; four-way junction DNA binding; glycolipid binding; heparin binding; open form four-way junction DNA binding; peptide binding; phosphatidylserine binding; protein dimerization activity; protein kinase activator activity; repressing transcription factor binding; sequence-specific DNA binding transcription factor activity; single-stranded DNA binding; transcription factor binding;
Pathway	Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem;

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