Human HMGB1 HEK293 Cell Lysate

Name: Human HMGB1 HEK293 Cell Lysate
SKU: CLOE-1521
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1521 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1521
Introduction	This cell lysate over-expresses the human HMGB1 (NP_002119.1) (Met 1-Glu 215) fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	HMGB1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	HMGB1 high mobility group box 1 [ Homo sapiens ]
Gene Symbol	HMGB1
Synonyms	HMG1; HMG3; SBP-1
Gene Description	high-mobility group box 1
Gene ID	3146
Uni Prot ID	P09429
m RNA Refseq	NM_002128.4
Protein Refseq	NP_002119.1
Chromosome Location	13q12
Function	DNA binding, bending; DNA binding, bending; RAGE receptor binding; calcium-dependent protein kinase regulator activity; chemoattractant activity; cytokine activity; damaged DNA binding; double-stranded DNA binding; protein binding; protein kinase activator activity; repressing transcription factor binding; sequence-specific DNA binding transcription factor activity; single-stranded DNA binding; transcription factor binding;
Pathway	Activated TLR4 signalling, organism-specific biosystem; Activation of DNA fragmentation factor, organism-specific biosystem; Advanced glycosylation endproduct receptor signaling, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis induced DNA fragmentation, organism-specific biosystem; Apoptotic execution phase, organism-specific biosystem;
MIM	163905

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