Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2835 Host Cell : HEK293
| Cat. No. | CLOE-2835 |
| Introduction | This cell lysate overexpresses mouse Ela2 (Met1-Arg260) with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Ela2 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Elane elastase, neutrophil expressed [ Mus musculus ] |
| Gene Symbol | Ela2 |
| Synonyms | NE; Ela2; F430011M15Rik |
| Gene Description | elastase 2, neutrophil |
| Gene ID | 50701 |
| Uni Prot ID | Q3UP87 |
| m RNA Refseq | NM_015779.2 |
| Protein Refseq | NP_056594.2 |
| Chromosome Location | 10 C1; 10 43.0 cM |
| Function | catalytic activity; cytokine binding; endopeptidase activity; heparin binding; hydrolase activity; peptidase activity; protease binding; serine-type endopeptidase activity; serine-type peptidase activity; |
| Pathway | Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Systemic lupus erythematosus, organism-specific biosystem; Systemic lupus erythematosus, conserved biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem; |
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