Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2779 Host Cell : HEK293
| Cat. No. | CLOE-2779 |
| Introduction | This cell lysate overexpresses mouse Angpt1 (Arg277-Phe498) with a N-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Angpt1 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Angpt1 angiopoietin 1 [ Mus musculus ] |
| Gene Symbol | Angpt1 |
| Synonyms | Ang1; Ang-1; 1110046O21Rik |
| Gene Description | angiopoietin 1 |
| Gene ID | 11600 |
| Uni Prot ID | O08538 |
| m RNA Refseq | NM_009640.3 |
| Protein Refseq | NP_033770.2 |
| Chromosome Location | 15 B3.1; 15 14.3 cM |
| Function | receptor binding; receptor tyrosine kinase binding; vascular endothelial growth factor receptor binding; |
| Pathway | Cell surface interactions at the vascular wall, organism-specific biosystem; HIF-1 signaling pathway, organism-specific biosystem; Hemostasis, organism-specific biosystem; PI3K-Akt signaling pathway, organism-specific biosystem; PI3K-Akt signaling pathway, conserved biosystem; Rheumatoid arthritis, organism-specific biosystem; Rheumatoid arthritis, conserved biosystem; |
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