Panoply™ Human ANGPT1 Over-expressing Stable Cell Line

Angiopoietin-1 (ANG1, also known as ANGPT1) is a pro-angiogenic regulatory factor that drives the progression of solid tumors by stimulating the proliferation, migration, and lumen formation of vascular endothelial cells, as well as by promoting vascular remodeling and stabilization. However, the function and underlying mechanisms of ANG1 in triple-negative breast cancer (TNBC) remain unclear. Clinical data indicate that elevated ANG1 levels in TNBC are associated with a poor prognosis compared to non-TNBC. Furthermore, ANG1 knockdown inhibits TNBC cell proliferation and induces G1 phase cell cycle arrest and apoptosis, whereas ANG1 overexpression promotes tumor growth in nude mice. Mechanistic studies reveal that ANG1 facilitates TNBC progression by upregulating the expression of carboxypeptidase A4 (CPA4). In summary, the ANG1-CPA4 axis represents a potential therapeutic target for TNBC.

To investigate the effect of ANG1 on triple-negative breast cancer (TNBC), researchers examined ANG1 expression in TNBC cell lines and selected four lines for subsequent study. They knocked down ANG1 in Hs578T and SUM149PT cells, which exhibit high ANG1 protein levels (Figure 1A), and overexpressed ANG1 in HCC1806 and MDA-MB-468 cells, which exhibit low ANG1 protein levels (Figure 1E). SRB and colony formation assays demonstrated that proliferation was inhibited in ANG1-knockdown Hs578T and SUM149PT cells (Figure 1B-D), whereas it was significantly promoted in ANG1-overexpressing HCC1806 and MDA-MB-468 cells (Figure 1F, G).

Figure 1. ANG1 promotes the proliferation of TNBC cells. (Liu X, et al., 2023)