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Human XRCC6 Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0057

Target Gene :   XRCC6 Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0057
Description HCT116-XRCC6 (+/-) is a cell line with a heterozygous knockout of human XRCC6
Target Gene XRCC6
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name XRCC6 X-ray repair complementing defective repair in Chinese hamster cells 6 [ Homo sapiens ]
Gene Symbol XRCC6
Synonyms ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Gene Description X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70kDa)
Gene ID 2547
Uni Prot ID B1AHC8
m RNA Refseq NM_001469.3
Protein Refseq NP_001460.1
Chromosome Location 22q13.2
Function 5-deoxyribose-5-phosphate lyase activity; ATP binding; ATP-dependent DNA helicase activity; DNA binding; double-stranded DNA binding; contributes_to double-stranded telomeric DNA binding; protein C-terminus binding; protein binding; transcription regulatory region DNA binding;
Pathway 2-LTR circle formation, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; DNA Repair, organism-specific biosystem; DNA-PK complex, organism-specific biosystem; Disease, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem;
MIM 152690
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Grateful

These knockout cell lines are fantastic! They've greatly improved the efficiency and accuracy of my experiments.

United States

Affordable

I'm so grateful for this brand's XRCC6 knockout cell line. They're affordable, reliable, and easy to use, making my experiments a success.

United States

Effective

This brand's XRCC6 knockout cell line are a game-changer! They're highly effective and have helped me achieve my research goals.

United States

Highly recommended

Thanks to these XRCC6 knockout cell line, my experiments have become more streamlined and efficient. I highly recommend them!

United States

Best, reliable

These XRCC6 knockout cell line are the best I've ever used! They're highly reliable and have greatly improved the accuracy of my results.

United States

Successful and must-have

Thanks to this brand's XRCC6 knockout cell line, my experiments have been more successful than ever before. They're a must-have for any researcher!

United States

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