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Human XIAP Knockout Cell Line-DLD-1

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0026

Target Gene :   XIAP Host Cell :   DLD-1

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0026
Description DLD-1-XIAP (-/Y) is a cell line with knockout of X-linked XIAP allele in male parental cells
Target Gene XIAP
Host Cell DLD-1
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name Homo sapiens X-linked inhibitor of apoptosis DNA.
Gene Symbol XIAP
Synonyms API3, ILP1, MIHA, XLP2, BIRC4
mRNA Refseq NM_001167.2
Quick Inquiry

Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Good comments

I read a lot of reviews before starting the transfection experiment and this one was relatively good and the price was right, so after trying it out I've already decided to follow up with it.

United States

Accurate results

I came across this brand of transfection cell line in the forum, and I read that some of the bigwigs who posted articles felt that this cell line was more accurate and the experimental data was more reliable.

United States

Looking forward to the results

My previous order of cell lines failed, the results were extremely erratic and wasted too much time, now I have ordered this cell line and hope it will not fail me, I will come back to update the follow-up evaluation.

United States

Good results

I did a lot of research before ordering this cell line, reading what other people had already posted about what cell lines they used, and finally ordered this one straight away, and it turned out fine.

United States

Clear trend

Good transfection efficiency, relatively time saving, experimental control is still very obvious, good results, visible trend to the naked eye.

United States

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