Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RT0026
Target Gene : XIAP Host Cell : DLD-1
Size : >1x106 cells/vial Validation : Sequencing
| Cat. No. | CSC-RT0026 |
| Description | DLD-1-XIAP (-/Y) is a cell line with knockout of X-linked XIAP allele in male parental cells |
| Target Gene | XIAP |
| Host Cell | DLD-1 |
| Host Cell Species | Homo sapiens (Human) |
| Size | >1x106 cells/vial |
| Validation | Sequencing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | Homo sapiens X-linked inhibitor of apoptosis DNA. |
| Gene Symbol | XIAP |
| Synonyms | API3, ILP1, MIHA, XLP2, BIRC4 |
| mRNA Refseq | NM_001167.2 |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
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I read a lot of reviews before starting the transfection experiment and this one was relatively good and the price was right, so after trying it out I've already decided to follow up with it.
I came across this brand of transfection cell line in the forum, and I read that some of the bigwigs who posted articles felt that this cell line was more accurate and the experimental data was more reliable.
My previous order of cell lines failed, the results were extremely erratic and wasted too much time, now I have ordered this cell line and hope it will not fail me, I will come back to update the follow-up evaluation.
I did a lot of research before ordering this cell line, reading what other people had already posted about what cell lines they used, and finally ordered this one straight away, and it turned out fine.
Good transfection efficiency, relatively time saving, experimental control is still very obvious, good results, visible trend to the naked eye.
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