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Human PTTG1 Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0052

Target Gene :   PTTG1 Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0052
Description HCT116-PTTG1 (-/-) is a cell line with a homozygous knockout of human PTTG1
Target Gene PTTG1
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name PTTG1 pituitary tumor-transforming 1 [ Homo sapiens ]
Gene Symbol PTTG1
Synonyms EAP1; PTTG; HPTTG; TUTR1
Gene Description pituitary tumor-transforming 1
Gene ID 9232
Uni Prot ID O95997
m RNA Refseq NM_004219.2
Protein Refseq NP_004210.1
Chromosome Location 5q35.1
Function SH3 domain binding; cysteine-type endopeptidase inhibitor activity; heat shock protein binding; protein binding; ribosome binding; sequence-specific DNA binding transcription factor activity;
Pathway APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem;
MIM 604147
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Progressing well

When conducting gene knockout experiments, this brand's cell lines are my top choice. They provide outstanding results and make my experiments smoother and more efficient.

United States

Successful experiments

This PTTG1 knockout cell line are amazing! Thank you for making my experiments more successful.

United States

Reliable affordable

I'm so impressed with this brand's PTTG1 knockout cell line - they're reliable, affordable, and easy to use.

United States

Accurate results

Thanks to these PTTG1 knockout cell line, I've achieved more accurate and consistent experimental results.

United States

Streamline experiments

This brand's PTTG1 knockout cell line are a game-changer! Thank you for helping me streamline my experiments.

United States

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