Human NHEJ1 (+/-) Knockout Cell Line-HCT116

Name: Human NHEJ1 (+/-) Knockout Cell Line-HCT116
SKU: CSC-RT0037
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0037

Target Gene : NHEJ1 Host Cell : HCT116

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0037
Description	HCT116-NHEJ1 (+/-) is a cell line with a heterozygous knockout of human NHEJ1
Target Gene	NHEJ1
Host Cell	HCT116
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NHEJ1 nonhomologous end-joining factor 1 [ Homo sapiens ]
Gene Symbol	NHEJ1
Synonyms	XLF
Gene ID	79840
Uni Prot ID	Q9H9Q4
m RNA Refseq	NM_024782.2
Protein Refseq	NP_079058.1
Chromosome Location	2q35
Function	DNA binding; protein binding;
Pathway	Non-homologous end joining, organism-specific biosystem; Non-homologous end-joining, organism-specific biosystem; Non-homologous end-joining, conserved biosystem;
MIM	611290

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Will also buy

I am going to use this brand of transfection reagent for my future experiments and I am going to use it in the future as I got satisfactory results in this one experiment.

United States

2023-01-16

Good transfer

I was even sceptical before I did the experiment, I thought it was because my experimental technique was not good, I tried it and found that surprisingly the transfection was not bad.

United States

2023-02-22

Saves time

This reagent is similar to the one I have used before, it is not cumbersome and the process is not very long, which saves time.

United States

2023-03-17

Saves energy

This reagent has saved a lot of time as I have used other reagents before with no satisfactory results or even said there was no trend, this one is different and the results are very consistent.

United States

2023-03-12

Second purchase

This is the reagent company recommended to me by my colleague. I have used other reagent trial kits before and the knockout reagent transfection for this build is also very good and I plan to order it frequently in the future.

United States

2023-03-19

Good results

I finally got the results this time, and luckily I was able to change the reagents in time to have the results reported at the group meeting.

United States

2023-03-11

Fast progress

This product has saved a lot of time, two students on two subjects, one student using this brand and the other using an expensive brand, they are both almost at the same pace.

United States

2023-05-05

Recommend

I was recommended to this brand build the first time I used a knockout product and my colleagues are extremely positive about this brand, so hopefully it will help with my subject as well.

United States

2023-03-15

Better value

I experimented with this transfection cell line as soon as I received it and it was very successful on the first try, and after four repetitions I had credible results.Overall this cell line is better value.

United States

2023-02-14

Knockout clean

This is the first time I have used a cell line that completely knocked out a gene, I had previously used an overexpressed transfection virus and it didn't feel very obvious, this cell knocked out very cleanly.

United States

2023-01-17

Simple steps

The procedure is no different from that of my previous virus transfection tool and requires no additional steps, so it is a very hassle-free transfection tool.

United States

2023-03-18

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Human NHEJ1 (+/-) Knockout Cell Line-HCT116

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Will also buy

Good transfer

Saves time

Saves energy

Second purchase

Good results

Fast progress

Recommend

Better value

Knockout clean

Simple steps

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