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Human NHEJ1 (-/-) Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0049

Target Gene :   NHEJ1 Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0049
Description HCT116-NHEJ1 (-/-) is a cell line with a homozygous knockout of human NHEJ1
Target Gene NHEJ1
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name NHEJ1 nonhomologous end-joining factor 1 [ Homo sapiens ]
Gene Symbol NHEJ1
Synonyms XLF
Gene ID 79840
Uni Prot ID Q9H9Q4
m RNA Refseq NM_024782.2
Protein Refseq NP_079058.1
Chromosome Location 2q35
Function DNA binding; protein binding;
Pathway Non-homologous end joining, organism-specific biosystem; Non-homologous end-joining, organism-specific biosystem; Non-homologous end-joining, conserved biosystem;
MIM 611290
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Customer service expertise

I like the customer service of this brand very much. Their customer service staff are always very friendly and professional, able to answer my questions and provide support in a timely manner. This makes me feel very comfortable and relieved when using their cell lines.

United States

Cheap and easy to use

The price of this brand of NHEJ1 knockout cell line is very reasonable. I believe that their products are worth the money, and I am very happy to pay a reasonable price for high-quality cell lines.

United States

Efficient and stable

If you are looking for an efficient, stable and affordable NHEJ1 knockout cell line, I highly recommend you to try this brand of product.

United States

High experimental efficiency

This brand of knockout cell lines is perfect for my research needs. Their cell lines allow for fast and accurate knockout of target genes, making my experiments much more efficient. The cell lines are very stable and I have rarely experienced any experimental failures.

United States

Save money

The price of this brand of NHEJ1 knockout cell line is very reasonable. The quality of their cell lines is very good, and the price is not too high. I think this is a very value-for-money cell line, which can save a lot of costs for my research.

United States

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