Human NECTIN2 Stable Cell Line - CHO-K1

Name: Human NECTIN2 Stable Cell Line - CHO-K1
SKU: CSC-RO0201
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0201

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0201
Description	This cell line is engineered to stably overexpress the human NECTIN2 in CHO-K1 cells.
Introduction	This cell line is constructed by transfection of human nectin cell adhesion molecule 2 (NECTIN2) into CHO-K1, followed by stable cell selection. The expression of NECTIN2 has been analyzed through flow cytometry.
Target Gene	NECTIN2
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

What is the genetic background of the Human NECTIN2 Stable Cell Line - CHO-K1, and how does it contribute to its utility in research?

A: The Human NECTIN2 Stable Cell Line - CHO-K1 is derived from the Chinese Hamster Ovary (CHO) cell line, specifically the K1 subclone. This cell line has been genetically modified to stably express the human NECTIN2 gene, which encodes a cell adhesion molecule involved in various cellular processes. The genetic background of CHO-K1, with its well-characterized and relatively simple genome, makes it an ideal host for the expression of human proteins, including NECTIN2, for research purposes.

How does the Human NECTIN2 Stable Cell Line - CHO-K1 facilitate the study of cell adhesion and signaling pathways?

A: The cell line's stable expression of NECTIN2 allows researchers to investigate the role of this protein in cell adhesion, signaling, and immune responses. It provides a controlled system to study the interactions of NECTIN2 with its ligands and downstream signaling pathways, contributing to a better understanding of cell-cell interactions and their implications in health and disease.

What are the ethical considerations when using the Human NECTIN2 Stable Cell Line - CHO-K1 in research?

A: Ethical considerations include ensuring that the cell line is obtained from reputable sources, that the genetic modifications do not cause undue harm to the cells, and that the research adheres to guidelines for the use of animal-derived materials. Additionally, any research involving human genetic material must comply with relevant ethical standards and regulations.

How does the Human NECTIN2 Stable Cell Line - CHO-K1 contribute to drug discovery and development?

A: The cell line can be used to screen for compounds that modulate NECTIN2 activity, aiding in the discovery of new drugs for diseases where NECTIN2 plays a role, such as cancer and infectious diseases. It provides a platform for high-throughput screening and functional assays to identify potential therapeutic targets.

What are the biosecurity measures associated with handling the Human NECTIN2 Stable Cell Line - CHO-K1?

A: Biosafety measures include handling the cells in a sterile environment, using appropriate personal protective equipment (PPE), and disposing of waste materials according to local regulations. Additionally, the cell line should be stored and handled in a manner that prevents contamination and ensures the integrity of the genetic material.

How does the Human NECTIN2 Stable Cell Line - CHO-K1 aid in the investigation of immune cell interactions?

A: The cell line can be used to study the role of NECTIN2 in immune cell interactions, particularly in T cell signaling, where it acts as both a costimulator and a coinhibitor. This dual role can provide insights into the regulation of immune responses and the development of immune-based therapies.

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