Human LIG4 (-/-) Knockout Cell Line-HCT116

Name: Human LIG4 (-/-) Knockout Cell Line-HCT116
SKU: CSC-RT0036
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0036

Target Gene : LIG4 Host Cell : HCT116

Size : >1x10⁶ cells/vial Validation : Sequencing

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0036
Description	HCT116-LIG4 (-/-) is a cell line with a homozygous knockout of human LIG4
Target Gene	LIG4
Host Cell	HCT116
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LIG4 ligase IV, DNA, ATP-dependent [ Homo sapiens ]
Gene Symbol	LIG4
Gene Description	ligase IV, DNA, ATP-dependent
Gene ID	3981
Uni Prot ID	P49917
m RNA Refseq	NM_002312.3
Protein Refseq	NP_002303.2
Chromosome Location	13q33-q34
Function	ATP binding; DNA binding; DNA ligase (ATP) activity; DNA ligase activity; DNA ligase activity; ligase activity; metal ion binding; protein C-terminus binding; protein binding;
Pathway	2-LTR circle formation, organism-specific biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Early Phase of HIV Life Cycle, organism-specific biosystem; HIV Infection, organism-specific biosystem; HIV Life Cycle, organism-specific biosystem;
MIM	601837

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Customer Reviews

High efficiency

Finally the results are in and I'm not a lab junkie now, the transfections are good and crucially not expensive.

United States

2023-03-22

Good results

I have used this company to knock out other genes before and it is really good, I will recommend it to others

United States

2023-03-25

Save times

Regarding the AKT2 knockout experiment, I chose the right knockout reagent when I first approached it, so the experiment was done relatively quickly.

United States

2023-01-29

Easy to knock out

The gene was relatively easy to knock out and the reagents from the company I chose were reliable.

United States

2023-02-10

Cheap and easy to use

At the beginning I wanted to save money and bought a brand name, but all the money was wasted, and then I ordered an expensive one with a larger dosage, which burned more money, so I didn't order it again.

United States

2020-01-12

Good results

My student is using this cell line and I am satisfied with the results he has given, many replicates, and the transfection seems to be stable.

United States

2021-04-11

Use less

This cell line is much more versatile than the different functions I ordered before, and once I bought it and split it up, the results were good and I think it can support two subjects doing transfections.

United States

2021-11-21

Building stability

It's amazing, this cell line can be said to be particularly stable, one night the laboratory power failure, low temperature refrigerator overnight without power, the next day rushed to the transfection, the results can still knock out, really stable.

United States

2022-01-31

Save money

This knockout cell line was a big part of my project design and I am very happy to find that I am now using this company's product, which is cheap and cost effective and has very clear results.

United States

2022-08-05

Attractive prices

I have tried this knockout cell line and the result is very good, after that I plan to use his brand of antibody, the price is very attractive, looking forward to the result.

United States

2023-03-15

High transfection efficiency

I have been using this product for a total of two weeks so far and have already produced some results, excluding my experimental methods, the transfection efficiency of this product is quite high.

United States

2023-04-06

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Related Products

Cat.No.	Product Name	Price
CSC-RK0062	Nos3 Knockout Mouse Liver Cells	Inquiry
CSC-RK0064	Nos3 Knockout Mouse Lung Cells	Inquiry
CSC-RK0111	Cybb Knockout Mouse Bone Marrow Mesenchymal Stem Cells	Inquiry
CSC-RK0077	Tlr4 Knockout Mouse Embryonic Stem Cells	Inquiry
CSC-RK0100	Icam1 Knockout Mouse Spleen Cells	Inquiry
CSC-RK0053	Nos3 Knockout Mouse Lung Fibroblasts	Inquiry
CSC-RK0110	Cybb Knockout Mouse Bone Marrow Neutrophils	Inquiry
CSC-RK0081	Tlr4 Knockout Mouse Kidney Cells	Inquiry
CSC-RK0132	Ncf1 Knockout Mouse Liver Cells	Inquiry
CSC-RK0076	Tlr4 Knockout Mouse Embryonic Fibroblasts	Inquiry

Human LIG4 (-/-) Knockout Cell Line-HCT116

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

High efficiency

Good results

Save times

Easy to knock out

Cheap and easy to use

Good results

Use less

Building stability

Save money

Attractive prices

High transfection efficiency

Related Products

Related Services