Panoply™ Human LIG4 Knockdown Stable Cell Line

Name: Panoply™ Human LIG4 Knockdown Stable Cell Line
SKU: CSC-DC008699
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC008699

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Cell Line Information

Safety and Packaging

Gene Information

Cat. No.	CSC-DC008699
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	LIG4
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LIG4 ligase IV, DNA, ATP-dependent [ Homo sapiens ]
Gene Symbol	LIG4
Gene Description	ligase IV, DNA, ATP-dependent
Gene ID	3981
Uni Prot ID	P49917
m RNA Refseq	NM_002312.3
Protein Refseq	NP_002303.2
Chromosome Location	13q33-q34
Function	ATP binding; DNA binding; DNA ligase (ATP) activity; DNA ligase activity; DNA ligase activity; ligase activity; metal ion binding; protein C-terminus binding; protein binding;
Pathway	2-LTR circle formation, organism-specific biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Early Phase of HIV Life Cycle, organism-specific biosystem; HIV Infection, organism-specific biosystem; HIV Life Cycle, organism-specific biosystem;
MIM	601837

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Q & A

Customer Reviews

Customer Q&As

What is LIG4？

A: The LIG4 gene is an important repair gene in the non-homologous end joining (NHEJ) repair pathway, and plays an important role in the repair of DSBs.

What is the role of LIG4?

A: The protein encoded by this gene is a DNA ligase that links single-strand breaks in double-stranded polydeoxyribonucleotides in an ATP-dependent reaction This protein is key to v(d)j recombination and dna double-strand break (dsb) repair.

What adverse outcomes can occur with a defective LIG4 gene?

A: Defects or inactivation of the LIG4 gene reduce the DNA repair capacity of the NHEJ pathway, decreasing genomic integrity and inducing tumourigenesis.

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