Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO0230
Host Cell : GS-J1 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO0230 |
| Description | This cell line is engineered to stably overexpress the human CD28 in GS-J1 cells. |
| Introduction | GS-J1/CD28 cell line stably expresses human CD28 molecule. The functional validation of this cell line has been measured by cell-based anti-CTLA4 activity assay. |
| Target Gene | CD28 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | GS-J1 |
| Host Cell Species | Homo sapiens (Human) |
| Source | GS-J1 |
| Applications |
1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | Homo sapiens CD28 molecule DNA. |
| Gene Symbol | CD28 |
| Synonyms | Tp44; MGC138290; CD28 |
| m RNA Refseq | NM_006139.2 |
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