Human BLM Knockout Cell Line-HCT116

Name: Human BLM Knockout Cell Line-HCT116
SKU: CSC-RT0044
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0044

Target Gene : BLM Host Cell : HCT116

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0044
Description	HCT116-BLM (-/-) is a cell line with a homozygous knockout of human BLM
Target Gene	BLM
Host Cell	HCT116
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BLM Bloom syndrome, RecQ helicase-like [ Homo sapiens ]
Gene Symbol	BLM
Synonyms	BS; RECQ2; RECQL2; RECQL3
Gene ID	641
Uni Prot ID	P54132
m RNA Refseq	NM_000057.2
Protein Refseq	NP_000048.1
Chromosome Location	15q26.1
Function	ATP binding; ATP-dependent 3-5 DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATPase activity; G-quadruplex DNA binding; bubble DNA binding; four-way junction helicase activity; helicase activity; p53 binding; protein binding; single-stranded DNA binding;
Pathway	BRCA1-associated genome surveillance complex (BASC), organism-specific biosystem; Blooms syndrome complex, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Homologous recombination, organism-specific biosystem; Homologous recombination, conserved biosystem; Integrated Breast Cancer Pathway, organism-specific biosystem;
MIM	604610

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Costly

On the one hand, I think the reagents work well, I've done two knock-outs that were both ideal, and on the other hand, the validation was very good.

United States

2023-01-29

Good effect

Fluorescence validation, western blot, flow cytometry were done and the results were more uniform and the trends were more complete, this reagent is still quite efficient.

United States

2023-03-29

Result clear

The actual transfection is good, the protein expression is good, I have done my experiments on protein using this reagent, it saves a lot of time

United States

2023-02-17

Will continue to use

I have used antibodies before, and they are cheap and work well.This company is still trusted for its reagents.

United States

2023-03-02

Convinced

I didn't know about this company before and this time I used it because my teacher recommended it and I really spent a lot of money that I didn't need to spend before.

United States

2023-03-26

Convenient to use

This gene Knock-out cell line is very convenient to use, and the instruction book is very easy to understand. The knockout ability is stable, can guarantee my results reliability and repeatability.

United States

2020-01-12

Accurate results

This brand of BLM knockout cell line is very suitable for the purpose of my research. I have used their cell lines many times for experiments, and I can get very accurate data and results every time. These results have helped me a lot in my further research.

United States

2021-04-11

Good customer service

In addition to the high quality of cell lines, the brand's customer service is also excellent. Whenever I encounter problems or concerns, their customer service staff can always give me timely response and support.

United States

2021-11-21

Reasonably priced

The price of the brand's BLM knockout cell line is also very reasonable. Considering their high quality and practicality, I think the cell lines of this brand are good value for money.

United States

2022-01-31

Recommend

I highly recommend this brand of BLM knockout cell line to other researchers, and I believe they can also benefit from it.

United States

2022-08-05

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Human BLM Knockout Cell Line-HCT116

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Costly

Good effect

Result clear

Will continue to use

Convinced

Convenient to use

Accurate results

Good customer service

Reasonably priced

Recommend

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