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Wheat CRISPR/Cas9 Genome Editing Kit (Kanamycin)

For research use only. Not intended for any clinical use.
Cat.No.
CC-12
Description
The Wheat CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by TaU6 promoter, and a codon optimized Cas9 expression cassette driven by ubiquitin promoter. The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.
Applications
The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.
Efficiency
Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features
•Two functional components are combined into one vector which offers a higher transfection efficiency.
•High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and monocot codon optimized-Cas9.
•High activity of sgRNA in rice by using TaU6 (wheat U6) promoter.
•Mutiplex genome editing
Resistance
Kanamycin
Selection Marker
Kanamycin

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Q & A

Customer Reviews

Customer Q&As
What are the main components of CRISPR/Cas9?

A: CRISPR/Cas9 contains two major components: a sgRNA, which is responsible for recognizing target DNA, and the Cas9 endonuclease, which is responsible for generating DSB at predesigned target DNA site.

What is the difference between base editing and CRISPR?

A: Base editing, borrowed from CRISPR, is a precise genome editing approach. It generates targeted point mutations without DSBs, foreign donor templates, or HR.

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Customer Reviews
Simple Operation

Easy to operate, even for those who are not familiar with gene editing experiments.

United States

12/23/2022

Affordable

The Genome Editing Kit is affordable and is capable of multiple genome editing.

United States

12/24/2022

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