Wheat CRISPR/Cas9 Genome Editing Kit (GFP)

Name: Wheat CRISPR/Cas9 Genome Editing Kit (GFP)
SKU: CC-14
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-14

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Product Information

Cat. No.	CC-14
Description	The Wheat CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by TaU6 promoter, and a codon optimized Cas9 expression cassette driven by ubiquitin promoter. The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.
Efficiency	Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features	Two functional components are combined into one vector which offers a higher transfection efficiency. High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and monocot codon optimized-Cas9. High activity of sgRNA in rice by using TaU6 (wheat U6) promoter. Mutiplex genome editing
Applications	The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.
Resistance	Kanamycin
Selection Marker	GFP

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What is the genome size of wheat?

A: Compared with other major crops, the size of the genome of common wheat is very large (about 17 gigabytes, Gb), and each subgenome is about 5.5 Gb, with complex composition, of which more than 80% is composed of repetitive sequences.

How is CRISPR-Cas9 used in wheat?

A: In wheat, CRISPR/Cas-based genome editing has been applied to create favorable alleles in the form of null or altered functional mutations as well as various forms of heteroallelic combinations to improve a variety of agronomic traits, including grain quality, disease resistance, grain size, gluten content and preharvest sprouting.

Can gene editing improve wheat yield?

A: Yes, during yield-plot experiments in the field, the researchers discovered that the gene-edited mutant wheat had significantly more grains per spike compared with the wild type, thereby increasing yield per unit area.

Can gene editing improve wheat disease resistance?

A: Yes. Through the effective application of genome editing, a group of Chinese researchers developed a highly resistant wheat. In a study published online in Nature, Chinese scientists described their use of genome editing to achieve strong disease resistance without any growth defects.

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Customer Reviews

Great Kit

Great kit. Using Wheat CRISPR/Cas9 Genome Editing Kit, I successfully generated double- and triple-knockout mutations of a pollen-specific phospholipase gene, MTL.

United States

2022-09-10

Useful Tool

CRISPR-Cas9 technology has been used to target and knock out important genes in agriculture and science. This Wheat CRISPR/Cas9 Genome Editing Kit has provided great convenience for our research. I recommend it.

United States

2023-01-18

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Wheat CRISPR/Cas9 Genome Editing Kit (GFP)

Product Information

Q & A

Customer Reviews

What is the genome size of wheat?

How is CRISPR-Cas9 used in wheat?

Can gene editing improve wheat yield?

Can gene editing improve wheat disease resistance?

Great Kit

Useful Tool

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