STAT3-Luc Reporter Cell Line-Ba/F3

Name: STAT3-Luc Reporter Cell Line-Ba/F3
SKU: CSC-RR0481
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0481

Host Cell : Ba/F3 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RR0481
Description	STAT3-Luc Reporter Cell Line-Ba/F3 stably expresses luciferase reporter gene under the control of the STAT3 responsive promoter. This cell line is an ideal cellular model for monitoring STAT3 signaling pathway activities.
Target Gene	STAT3-Luc
Host Cell	Ba/F3
Host Cell Species	Mus musculus (Mouse)
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Applications

Q & A

Customer Reviews

The STAT3 (Signal Transducer and Activator of Transcription 3) gene encodes a transcription factor that plays a critical role in cell growth, differentiation, and immune responses. STAT3 is often constitutively activated in various cancers, contributing to tumor cell survival, proliferation, and resistance to apoptosis. Targeting STAT3 signaling is a promising strategy for cancer therapy.

The Ba/F3 cell line, as previously described, is a versatile model for studying oncogenic signaling. The STAT3-Luc reporter cell line in Ba/F3 cells is engineered to express a luciferase gene driven by STAT3-responsive promoters. This system allows for the monitoring of STAT3 activity in real-time, providing a powerful tool for studying STAT3 signaling pathways, the effects of potential therapeutic agents on STAT3 activity, and the development of targeted cancer treatments.

The STAT3-Luc Reporter Cell Line - Ba/F3 is a specialized tool for examining the Signal Transducer and Activator of Transcription 3 (STAT3) pathway, which is implicated in numerous cellular processes, including cell growth, differentiation, and immune responses.

(1)Immunology Research: STAT3 plays a critical role in immune cell function. This cell line can be used to study the activation of STAT3 in response to various cytokines and to investigate the downstream effects of STAT3 signaling in immune cell behavior.

(2)Cancer Biology: STAT3 is often hyperactivated in cancer, promoting tumor cell survival and proliferation. Researchers can use this cell line to identify and test compounds that disrupt STAT3 signaling as a potential cancer therapy strategy.

(3)Gene Expression Profiling: By measuring STAT3-dependent luciferase activity, this cell line allows for the assessment of the impact of different treatments or genetic modifications on STAT3-responsive gene expression, providing insights into the regulation of STAT3 target genes.

Customer Q&As

How can the STAT3-Luc Reporter Cell Line-Ba/F3 be used to investigate the efficacy of novel STAT3 inhibitors in preclinical research?

A: This cell line can be treated with candidate STAT3 inhibitors, and the inhibition of STAT3 activity can be assessed by measuring the reduction in luciferase activity. A decrease in luminescence indicates effective inhibition of the STAT3 signaling pathway.

What approach would you take to study the role of STAT3 in cytokine signaling using the STAT3-Luc Reporter Cell Line-Ba/F3?

A: By stimulating the cells with cytokines known to activate STAT3, such as IL-6 or IL-10, and measuring luciferase activity, researchers can elucidate the role of STAT3 in cytokine-induced signal transduction pathways.

In the context of immune response, how might the STAT3-Luc Reporter Cell Line-Ba/F3 serve to model the activation of STAT3 during inflammatory processes?

A: The cell line can be exposed to inflammatory stimuli like LPS or pro-inflammatory cytokines. The consequent STAT3 activation can be monitored via luciferase activity, providing insight into the transcriptional regulation of inflammatory genes mediated by STAT3.

Can the STAT3-Luc Reporter Cell Line-Ba/F3 be used to perform a time-course analysis of STAT3 transcriptional activity in response to growth factor stimulation?

A: Yes, by treating the cells with growth factors such as EGF or PDGF at different time points and measuring the luciferase activity over time, one can perform a kinetic analysis of STAT3 transcriptional activity in response to stimulation.

How would you validate the specificity of STAT3 activation in the STAT3-Luc Reporter Cell Line-Ba/F3 when analyzing the effects of pharmacological agents?

A: To validate specificity, one can use STAT3-specific siRNA or CRISPR/Cas9-mediated gene editing to knock down or knock out STAT3 in the cell line. A corresponding decrease in luciferase activity following treatment with pharmacological agents would indicate specificity to the STAT3 pathway. Additionally, using inhibitors known to act on other STAT proteins can help demonstrate the specificity of agents for STAT3.

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Customer Reviews

High Sensitivity to STAT3 Activation

The STAT3-Luc Reporter Cell Line-Ba/F3 is engineered to provide high sensitivity to STAT3 activation. This means that even minimal changes in STAT3 activity are detectable, offering precise insights into STAT3 signaling dynamics within biological research.

Germany

2022-01-01

Rapid Response to Stimuli

This cell line reacts quickly to external stimuli that activate STAT3, allowing us to observe STAT3 activity in real-time. Rapid responses facilitate timely data collection and analysis, crucial for experiments requiring immediate results.

Canada

2022-06-12

Easy to Culture

STAT3-Luc Reporter Cell Line-Ba/F3 grows under standard cell culture conditions, making it easy to maintain and use in the lab. This advantage reduces the need for specialized training or equipment, thus lowering laboratory costs and complexities.

United Kingdom

2020-09-13

Stable Reporter Integration

The luciferase reporter gene in this cell line is stably integrated, ensuring consistent and reliable readouts across different experiments and prolonged studies. This stability is crucial for longitudinal studies where variations in reporter expression could affect data integrity.

Germany

2021-11-19

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STAT3-Luc Reporter Cell Line-Ba/F3

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Applications

Q & A

Customer Reviews

How can the STAT3-Luc Reporter Cell Line-Ba/F3 be used to investigate the efficacy of novel STAT3 inhibitors in preclinical research?

What approach would you take to study the role of STAT3 in cytokine signaling using the STAT3-Luc Reporter Cell Line-Ba/F3?

In the context of immune response, how might the STAT3-Luc Reporter Cell Line-Ba/F3 serve to model the activation of STAT3 during inflammatory processes?

Can the STAT3-Luc Reporter Cell Line-Ba/F3 be used to perform a time-course analysis of STAT3 transcriptional activity in response to growth factor stimulation?

How would you validate the specificity of STAT3 activation in the STAT3-Luc Reporter Cell Line-Ba/F3 when analyzing the effects of pharmacological agents?

High Sensitivity to STAT3 Activation

Rapid Response to Stimuli

Easy to Culture

Stable Reporter Integration

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