Cat.No. : CSC-RR0481 Host Cell: Ba/F3
| Cat. No. | CSC-RR0481 |
| Description | STAT3-Luc Reporter Cell Line-Ba/F3 stably expresses luciferase reporter gene under the control of the STAT3 responsive promoter. This cell line is an ideal cellular model for monitoring STAT3 signaling pathway activities. |
| Gene | STAT3-Luc |
| Host Cell | Ba/F3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line can be treated with candidate STAT3 inhibitors, and the inhibition of STAT3 activity can be assessed by measuring the reduction in luciferase activity. A decrease in luminescence indicates effective inhibition of the STAT3 signaling pathway.
A: By stimulating the cells with cytokines known to activate STAT3, such as IL-6 or IL-10, and measuring luciferase activity, researchers can elucidate the role of STAT3 in cytokine-induced signal transduction pathways.
A: The cell line can be exposed to inflammatory stimuli like LPS or pro-inflammatory cytokines. The consequent STAT3 activation can be monitored via luciferase activity, providing insight into the transcriptional regulation of inflammatory genes mediated by STAT3.
A: Yes, by treating the cells with growth factors such as EGF or PDGF at different time points and measuring the luciferase activity over time, one can perform a kinetic analysis of STAT3 transcriptional activity in response to stimulation.
A: To validate specificity, one can use STAT3-specific siRNA or CRISPR/Cas9-mediated gene editing to knock down or knock out STAT3 in the cell line. A corresponding decrease in luciferase activity following treatment with pharmacological agents would indicate specificity to the STAT3 pathway. Additionally, using inhibitors known to act on other STAT proteins can help demonstrate the specificity of agents for STAT3.
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The STAT3-Luc Reporter Cell Line-Ba/F3 is engineered to provide high sensitivity to STAT3 activation. This means that even minimal changes in STAT3 activity are detectable, offering precise insights into STAT3 signaling dynamics within biological research.
This cell line reacts quickly to external stimuli that activate STAT3, allowing us to observe STAT3 activity in real-time. Rapid responses facilitate timely data collection and analysis, crucial for experiments requiring immediate results.
STAT3-Luc Reporter Cell Line-Ba/F3 grows under standard cell culture conditions, making it easy to maintain and use in the lab. This advantage reduces the need for specialized training or equipment, thus lowering laboratory costs and complexities.
The luciferase reporter gene in this cell line is stably integrated, ensuring consistent and reliable readouts across different experiments and prolonged studies. This stability is crucial for longitudinal studies where variations in reporter expression could affect data integrity.
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