Unlike mRNAs, small RNAs lack poly-A tails. Therefore, cloning small RNA molecules begins with ligation of adaptors to the 5′ and 3′ ends of small RNAs followed by reverse transcription and PCR amplification. This kit contains all of the reagents necessary for the isolation, cDNA synthesis, and amplification steps that allow cloning of small (18-26 bases) RNA molecules (including microRNAs).
Efficient cDNA synthesis from small RNAs (18-26 bases)
•Small RNA cloning for preparing small RNA libraries •Real-Time PCR •cDNA can be used for TA cloning and small RNA library generation
Package with enzyme should be stored at -20°C, others can be stored at 4°C.