small RNA Cloning Kit

Name: small RNA Cloning Kit
SKU: RTSZ-01
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : RTSZ-01

Inquire for Price

Product Information

Cat. No.	RTSZ-01
Description	Unlike mRNAs, small RNAs lack poly-A tails. Therefore, cloning small RNA molecules begins with ligation of adaptors to the 5′ and 3′ ends of small RNAs followed by reverse transcription and PCR amplification. This kit contains all of the reagents necessary for the isolation, cDNA synthesis, and amplification steps that allow cloning of small (18-26 bases) RNA molecules (including microRNAs).
Sample Type	RNA
Report Gene	Small RNAs to cDNA
Features	Efficient cDNA synthesis from small RNAs (18-26 bases)
Applications	•Small RNA cloning for preparing small RNA libraries •Real-Time PCR •cDNA can be used for TA cloning and small RNA library generation
Selection Marker	RNA
Storage	Package with enzyme should be stored at -20°C, others can be stored at 4°C.
Shipping	Dry ice

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What are the components of the kit?

A: This kit contains all of the reagents necessary for the isolation, cDNA synthesis, and amplification steps that allow cloning of small (18-26 bases) RNA molecules (including microRNAs).

How is it different from cloning mRNA?

A: Cloning and analyzing small RNA molecules is one method used to assess their expression patterns. Unlike mRNAs, small RNAs lack poly-A tails. Therefore, cloning small RNA molecules involves ligation of RNA or RNA/DNA chimeric adaptors to the 5’ and 3’ ends of extracted small RNAs followed by reverse transcription and then PCR.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Customer Reviews

This Kit Provides A Simplified Workflow.

By using magnetic beads, this kit provides a simplified workflow that does not require gel extraction of nucleic acids after adaptor ligation. Therefore, small RNA-derived cDNAs can be amplified by a simple procedure. The amplified cDNA can then be used directly for TA cloning.

United States

2022-12-03

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Related Products

Cat.No.	Product Name	Price
RTFZ-02	First Strand cDNA Synthesis Kit(With gDNase)	Inquiry
RTCZ-01	Cells-to-cDNA Kit	Inquiry
RTFZ-03	LA First Strand cDNA Synthesis Kit	Inquiry
RTFZ-01	First Strand cDNA Synthesis Kit	Inquiry
RTMZ-01	miRNA First-strand cDNA synthesis kit	Inquiry

small RNA Cloning Kit

Product Information

Q & A

Customer Reviews

What are the components of the kit?

How is it different from cloning mRNA?

This Kit Provides A Simplified Workflow.

Related Products

Related Services