First Strand cDNA Synthesis Kit

Name: First Strand cDNA Synthesis Kit
SKU: RTFZ-01
Rating: 4.0 (1 reviews)

For research use only. Not intended for any clinical use.

Cat.No.

RTFZ-01

Description

This kit is designed for the first strand cDNA synthesis using a wide range of total RNA or poly(A)+ RNA amounts. The Reverse Transcriptase in this kit can read-through of templates with high GC content or complex secondary structures.

Applications

•RT-PCR (Endpoint)
•Real-Time PCR

Features

High specificity and sensitivity

Report Gene

First strand cDNA synthesis

Sample Type

RNA

Selection Marker

RNA

Shipping

Dry ice

Storage

Store at -20°C

Publications

Q & A

Customer Reviews

Customer Q&As

For reverse transcription, how important is the quality of RNA template?

A: RNA purity and integrity are essential for the synthesis and quantification of cDNA. Always assess the integrity of RNA prior to cDNA synthesis. Use freshly prepared RNA. Multiple freeze/thaw cycles of the RNA sample and synthesized cDNA are not recommended. Avoid RNase contamination and discard low-quality RNA.

What steps should I take while performing first strand cDNA synthesis using low purity template (e.g., inhibitors in RNA sample)?

A: Trace amounts of reagents used in RNA purification protocols may remain in solution and inhibit first-strand synthesis, e.g., SDS, EDTA, guanidine salts, phosphate, pyrophosphate, polyamines, and spermidine. We recommend re-precipitating the RNA with ethanol and washing the pellet with 75% ethanol or re-purifying the RNA to remove trace contaminants.

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Customer Reviews

The Kit Was Very Specific And Has High Sensitivity.

I have used Creative Biogene's First Strand cDNA Synthesis Kit for the synthesis of cDNA using random hexamers as primer with E. coli RNA samples to measure gene expression by quantitative real-time PCR. The kit was very specific and has high sensitivity in the synthesis of cDNA. Started with 1 microgram of total RNA as input and got around 4 to 5 micrograms of cDNA which was sufficient enough for my all qPCR assays.

United States

12/03/2022

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First Strand cDNA Synthesis Kit

Publications

Q & A

Customer Reviews

For reverse transcription, how important is the quality of RNA template?

What steps should I take while performing first strand cDNA synthesis using low purity template (e.g., inhibitors in RNA sample)?

The Kit Was Very Specific And Has High Sensitivity.

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