Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : RTCZ-01
| Cat. No. | RTCZ-01 |
| Description | The kit provides a fast and simple procedure for preparing first-strand cDNA directly from cultured cells without RNA purification. The kit is supplied with all reaction components for fast and efficient cDNA synthesis. |
| Sample Type | Cell |
| Report Gene | Cells to cDNA directly |
| Features |
•Simple Procedure: The kit comes with everything you need to go from cultured cells to PCR-ready cDNA; no RNA isolation is required. •Ideal for experiments that require rapid, high-throughput gene expression analysis, such as: Validation of siRNA-mediated gene knockdown; Screening of potential drugs; Detection of stimulated gene regulation. •The kit is compatible with RT-PCR protocols. |
| Applications |
•RT-PCR (Endpoint) •Real-Time PCR |
| Selection Marker | Cells |
| Storage | Store at -20°C |
| Shipping | Dry ice |
A: Yes, we offer our Cells-to-cDNA Kit that allow you to bypass RNA purification.
A: While we have not tested this in house, some papers did use our kit on cells infected with hepatitis C virus.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Cells-to-cDNA Kit has performed consistently. The performance is increased as compared to RNA purification, RT steps, and then qRT-PCR. There is less handling, less chance at mistakes.
This kit is ideal for performing RT-PCR on small numbers of cells, numerous cell samples, or for scientists who are unfamiliar with RNA isolation procedures. The kit is also adaptable to high-throughput analysis using 96-well plates. The cDNA produced is suitable for any PCR-related application including quantitative RT-PCR and cloning.
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