scFv(Control)-CD28-OX40-CD3zeta CAR-T Lentivirus

scFv(Control)-CD28-OX40-CD3ζ CAR-T Lentivirus is a matched control reagent developed for CAR-T-cell research. It encodes a chimeric antigen receptor containing a control single-chain variable fragment (scFv), membrane-anchoring elements, tandem intracellular costimulatory domains from CD28 and OX40 (CD134), and the CD3ζ activation domain. The control scFv is selected to lack relevant recognition of the target antigen used in the experimental system while preserving an overall receptor architecture comparable to that of an antigen-specific third-generation CAR. This design helps distinguish responses driven by antigen recognition from effects related to lentiviral transduction, receptor expression, or the signaling backbone. Lentiviral delivery supports efficient gene transfer and stable CAR expression in transduced T cells, providing a consistent control population for direct comparison with target-specific CAR-T cells.

This product is intended for use as a negative control in studies of antigen-dependent CAR-T-cell function. Control-transduced T cells can be compared with target-specific cells when assessing transduction efficiency, receptor expression, viability, proliferation, differentiation, basal activation, and expansion. In co-culture assays, they help establish background levels of target-cell killing, cytokine secretion, degranulation, and nonspecific responses to antigen-positive or antigen-negative cells. The construct is also useful for investigating tonic signaling, receptor clustering, activation-induced cell death, and phenotypic changes associated with prolonged CAR expression. In appropriately designed animal studies, the control group can help separate antigen-specific effects from responses caused by T-cell administration, vector exposure, ex vivo culture, or the disease model itself. Because the intracellular architecture includes both CD28 and OX40, this reagent is particularly suitable as a backbone-matched control for third-generation CAR constructs carrying the same signaling domains. Its lentiviral format supports reproducible control-cell preparation across screening, mechanistic, and preclinical research workflows.