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Rice CRISPR/Cas9 Genome Editing Kit (Kanamycin)

For research use only. Not intended for any clinical use.
Cat.No.
CC-09
Description
The Rice CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by OsU6 promoter, and a codon optimized Cas9 expression cassette driven by ubiquitin promoter. The Rice CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in rice.
Applications
The Rice CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in rice.
Efficiency
Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features
•Two functional components are combined into one vector which offers a higher transfection efficiency.
•High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and monocot codon optimized-Cas9.
•High activity of sgRNA in rice by using OsU6 (rice U6) promoter.
•Mutiplex genome editing
Resistance
Kanamycin
Selection Marker
Kanamycin

Publications

Q & A

Customer Reviews

Customer Q&As
Is this kit suitable for gene modification of other monocots?

A: The Rice CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots.

How can we achieve high expression of Cas9 protein in monocots?

A: High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and monocot codon optimized-Cas9.

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Customer Reviews
Economical and Effective

This kit helps me realize the simplest, most economical and most effective genome editing in rice.

United States

12/21/2022

Worked Very Well

We successfully used the Rice CRISPR/Cas9 Genome Editing Kit to target eIF4G in an RTSV-susceptible rice variety in an effort to develop resistance against rice tungro disease.

United States

12/18/2022

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