Rice CRISPR/Cas9 Genome Editing Kit (Hygromycin)

Name: Rice CRISPR/Cas9 Genome Editing Kit (Hygromycin)
SKU: CC-07
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-07

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Product Information

Cat. No.	CC-07
Description	The Rice CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by OsU6 promoter, and a codon optimized Cas9 expression cassette driven by ubiquitin promoter. The Rice CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in rice.
Efficiency	Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features	Two functional components are combined into one vector which offers a higher transfection efficiency. High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and monocot codon optimized-Cas9. High activity of sgRNA in rice by using OsU6 (rice U6) promoter. Mutiplex genome editing
Applications	The Rice CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in rice.
Resistance	Kanamycin
Selection Marker	Hygromycin B

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Customer Reviews

Customer Q&As

Why did my CRISPR plant construct did not produce the expected target cleavage?

A: Not every sgRNA will produce the target cleavage. It is recommended to use two to three sgRNAs per gene to increase the successful rate.

What antibiotics should I use for selection of the CRISPR rice vectors in Agrobacterium Plantarum?

A: Kanamycin.

What is the promoter used for the expression of Cas9?

A: A codon optimized Cas9 expression cassette is driven by ubiquitin promoter.

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Customer Reviews

Worked Very Well

This kit makes the system much simpler than other engineering nuclease systems. We have successfully knocked out a variety of rice genes, such as plant ene desaturase gene (OsPDS), betaine aldehyde dehydrogenase (OsBADH2), and mitogen-activated protein kinase (OsMPK2).

United States

2022-12-17

Time saving

Time saving. We soon completed the gene editing of rice.

United States

2022-12-18

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Rice CRISPR/Cas9 Genome Editing Kit (Hygromycin)

Product Information

Q & A

Customer Reviews

Why did my CRISPR plant construct did not produce the expected target cleavage?

What antibiotics should I use for selection of the CRISPR rice vectors in Agrobacterium Plantarum?

What is the promoter used for the expression of Cas9?

Worked Very Well

Time saving

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